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The Enteric and Parasitology Laboratory performs reference laboratory services to aid in the identification of enteric organisms submitted by laboratories throughout the state. This laboratory offers services to isolate, identify, and/or confirm Salmonella sp., Shigella sp., Campylobacter sp., and E. coli O157:H7, among others, from clinical specimens. Complete serotyping of Salmonella sp. and Shigella sp. is provided. Approved clients may also receive complete examination of paired formalin and polyvinyl alcohol (PVA) preserved stool specimens for intestinal parasites. Blood preparations for parasites are sent to the CDC for examination.
The Molecular Test Development Laboratory develops and implements methods for detection and subtyping of various gastrointestinal and respiratory pathogens. In addition, laboratory conducts specialized testing for selected pathogenic viruses and bacteria which are of public health interest. Polymerase chain reaction (PCR) amplification and detection of nucleic acids is used to identify E.coli 0157:H7 and Shigella sp in clinical specimens and contaminated food samples. Pulsed-field gel electrophoresis (PFGE) is performed to subtype isolates of foodborne pathogens such as Salmonella sp., E.coli 0157:H7, Shigella sp. and Listeria monocytogenes. Through PFGE, a unique bacterial DNA fingerprint is obtained, analyzed, and submitted to the PulseNet National Database at the Centers for Disease Control to track for local and national outbreak patterns. Chlamydia and Gonorrhea are detected from urine and genital specimens using a nucleic acid probe amplification assay.
The Mycology Laboratoryoffers identification services pertaining to all approved clinical, reference, and environmental isolates. Since most clinical and environmental samples are considered unique, approval prior to submission is necessary. Approval may be obtained through the laboratory supervisor at (317) 921-5500.
State law requires laboratories to submit isolates of Mycobacterium tuberculosis to the Mycobacteriology laboratory for confirmation and susceptibility testing. Drug susceptibility testing is routinely performed on all new cases of M. tuberculosis. Drug susceptibility testing will be repeated if the patient is still culture positive for M. tuberculosis after 3 months of therapy. Susceptibility testing for other Mycobacterial species is not performed. The laboratory also offers direct microscopic examination and culture of clinical specimens for primary identification of Mycobacterium species in cases referred by local health departments or the TB control program, (317) 233-7434.
The Reference Bacteriology Laboratory offers identification of selected bacterial pathogens from human sources which are of public health importance. Only pure viable cultures of clinically significant bacterial isolates are accepted. These include those of public health interest as well as those which Indiana State law requires to be submitted: Neisseria meningitides and Haemophilus influenzae from invasive disease, shiga-toxin producing E. coli, vancomycin-resistant Staphylococcus aureus, Listeria monocytogenes, and Salmonella sp. Isolates of Bordetella parapertussis, Bordetella pertussis, Legionella sp. and Corynebacterium diphtheriae from clinical specimens are accepted for confirmation. Grouping/typing is provided when appropriate. This laboratory is not a general identification service for laboratories that provide human clinical specimen testing. Obtain approval to submit isolates or specimens by calling ISDH Surveillance and Investigation at (317) 233-7125.
The Rabies Laboratory performs rabies testing Monday through Friday on a daily basis, excluding holidays. A complete description of how to submit specimens for rabies testing can be found on the back of the Rabies submission form or on the ISDH website. Results are usually available 24 hours after submission if the head/specimen is not frozen. Animal heads/specimens submitted on late Friday afternoon are held until Monday morning, or next business day. For further consultation regarding human exposure, call the ISDH Surveillance and Investigation at (317) 233-7272.
The Serology Laboratory offers serological analyses for infections of public health importance, including HIV, Hepatitis, and syphilis. It also tests for antibodies to infections associated with vector borne pathogens and other public health important pathogens such as arbovirus, coxiella, ehrlichia, rickettsia, hantavirus, legionella, influenza, mumps, rubella, rubeola and varicella.
The Virology Laboratory provides testing to detect and identify viruses pathogenic to humans which may be of public health importance. Some viruses are isolated in culture, e.g., respiratory viruses, herpes viruses causing skin lesions, enteroviruses, and mumps and measles viruses. Detection of nucleic acids in infected specimens is used to identify infections caused by mumps, influenza virus, and to subtype influenza isolates.
The Virology Laboratory also accepts reference viral isolates for confirmation and subtyping of viruses including influenza, enteroviruses, and adenoviruses. West Nile Virus is detected from pools of mosquitoes for surveillance data.
Food Microbiology Section isolates/identifies pathogens, foreign material, and insects in food products. The laboratory supports investigations of alleged food-borne illness incidents, consumer complaints, federally required routine surveillance of dairy products produced in Indiana, and required federal surveillance of the meat program for Salmonella. These investigations and monitoring programs are conducted for the citizens of Indiana by the Food Protection Division at the Indiana State Department of Health, local health departments, and the Indiana Board of Animal Health.
All samples submitted for a food, drug, or dairy analysis must be submitted through the Food Protection Division at the Indiana State Department of Health, or the Indiana Board of Animal Health. No specimens will be routinely accepted if sent directly to the laboratory.
Water Microbiology Section provides microbiological laboratory services required by the Indiana Department of Environmental Management (IDEM), Indiana Board of Animal Health (IBOAH), the ISDH Sanitary Engineering Division and the Food Protection Division and other state agencies and institutions. Basic EPA approved drinking water microbiological testing is also provided for a fee to public and private water suppliers and to individual Indiana citizens with residential wells. The routine tests include Total Coliform (P/A—MMO-MUG) , E.coli (P/A—MMO-MUG). E.coli (MF)and Heterotrophic Plate Count.